How to sync with EndNote Web

I’ve been syncing between desktop and web version for some time with no issues. Recently I moved my local endnote file to a new location, and this has broken the sync process. Under Sync Preferences, in the box labelled ‘Sync this endnote library’, it still lists the old file location even though I’ve opened the file from its new location. I assume that’s why it’s not syncing, but it doesn’t seem possible to change the location from within preferences (i.e., when you click on that box, you can’t do anything.) Thanks for any advice.