PMID- 20821044 OWN - NLM STAT- Publisher DA - 20100907 IS - 1573-7330 (Electronic) IS - 1058-0468 (Linking) DP - 2010 Sep 4 TI - Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study. AB - PURPOSE: To evaluate the meiotic spindle and chromosomal distribution of in vitro-matured oocytes from infertile nonobese women with PCOS and male or tubal causes of infertility (controls), and to compare in vitro maturation (IVM) rates between groups. METHODS: Seventy four patients (26 with PCOS and 48 controls) undergoing stimulated cycles of oocyte retrieval for ICSI were selected prospectively. Thirteen PCOS patients and 27 controls had immature oocytes retrieved submitted to IVM. After IVM, oocytes showing extrusion of the first polar body were fixed and processed for evaluation of the meiotic spindle and chromosome distribution by immunofluorescence microscopy. RESULTS: There were no differences between PCOS and control groups with respect to IVM rates (50.0% and 42.9%, respectively) nor the percentage of meiotic abnormalities in metaphase II oocytes (35.3% and 25%, respectively). CONCLUSIONS: In vitro-matured oocytes obtained from stimulated cycles of nonobese PCOS did not have an increased ratio of meiotic abnormalities. AD - Departamento de Ginecologia e Obstetricia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto, Sao Paulo, Brazil. AU - Vieira RC AU - Barcelos ID AU - Ferreira EM AU - Martins WP AU - Ferriani RA AU - Navarro PA LA - ENG PT - JOURNAL ARTICLE DEP - 20100904 TA - J Assist Reprod Genet JT - Journal of assisted reproduction and genetics JID - 9206495 EDAT- 2010/09/08 06:00 MHDA- 2010/09/08 06:00 CRDT- 2010/09/08 06:00 PHST- 2010/05/15 [received] PHST- 2010/08/23 [accepted] PHST- 2010/09/04 [aheadofprint] AID - 10.1007/s10815-010-9475-7 [doi] PST - aheadofprint SO - J Assist Reprod Genet. 2010 Sep 4. PMID- 20820887 OWN - NLM STAT- Publisher DA - 20100907 IS - 1567-2387 (Electronic) IS - 1567-2379 (Linking) DP - 2010 Sep 5 TI - Expression of ladybird-like homeobox 2 (LBX2) during ovarian development and folliculogenesis in the mouse. AB - The Ladybird-like homeobox gene 2 (Lbx2) belongs to the homeodomain-containing family of transcription factor that are known to play crucial role in various developmental processes. During early mouse embryogenesis, Lbx2 was shown to be expressed in the developing eye, brain and urogenital system. Although Lbx2 was detected in the testis and epididymis throughout development, no data was available regarding its expression in the female gonad. Here we have determined Lbx2 expression throughout mouse ovarian development by in situ hybridization. In contrast to the strong expression in the male fetal gonad, no Lbx2 signal could be detected in the fetal ovary. Soon after birth, however, Lbx2 expression was detected at different levels in various ovarian compartments (oocyte, granulosa cells, theca cells) where its expression was highly dynamic depending on the stage of follicular maturation. Our data would be consistent with a role for LBX2 in ovarian maturation and folliculogenesis. AD - Reproduction, Perinatal and Child Health, CHUQ Research Centre, CHUL Room T1-49, 2705 Laurier Blvd., Quebec City, QC, G1V 4G2, Canada, Vanessa.Moisan@crchul.ulaval.ca. AU - Moisan V AU - Robert NM AU - Tremblay JJ LA - ENG PT - JOURNAL ARTICLE DEP - 20100905 TA - J Mol Histol JT - Journal of molecular histology JID - 101193653 EDAT- 2010/09/08 06:00 MHDA- 2010/09/08 06:00 CRDT- 2010/09/08 06:00 PHST- 2009/11/08 [received] PHST- 2010/08/26 [accepted] PHST- 2010/09/05 [aheadofprint] AID - 10.1007/s10735-010-9291-5 [doi] PST - aheadofprint SO - J Mol Histol. 2010 Sep 5.